Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis

Figure 3

Effect of Angelica sinensis polysaccharides (APS-3c) on the expression of chondrocytes glycosyltransferases genes involved in glycosaminoglycan synthesis of rat interleukin-1-beta- (IL-1β-) stimulated chondrocytes. (a) RT-PCR of O-xylosyltransferase I (XylT-I), β1,4-galactosyltransferase 7 (GalT-I), β1,3-galactosyltransferase 6 (GalT-II), β1,3-glucuronosyltransferase I (GlcAT-I), N-acetylgalactosaminyltransferase 1 (Csgalnact-1), and GAPDH (from top to bottom) mRNA in response to various concentrations of APS-3c. Integrated density values for polymerase products were normalized to the values for GAPDH. ((b), (c), (d), (e), and (f)). The resulting data were expressed and illustrated as a ratio of the normal control. Values represent Mean ± SEM of four different simples. ^∗∗𝑃<0.01versus normal control. ^#𝑃<0.05, ^##𝑃<0.01versus IL-1β-stimulated control.