Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis

Table 1

Primer sequence and the optimal PCR conditions.

Gene	Accession number	Sequence	Tm (°C)	Cycle number	Product size (bp)
Aggrecan	NM_022190	F: 5′-CTAGCTGCTTAGCAGGGATAACG-3′	58	20	108
		R: 5′-TGACCCGCAGAGTCACAAAG-3′
Col2a1	NM_012929	F: 5′-GAGTGGAAGAGCGGAGACTACTG-3′	57	15	81
		R: 5′-CTCCATGTTGCAGAAGACTTTCA-3′
XylT-I	XM_341912	F: 5′-TTCACTGGGCGAGAGTGCAAGG-3′	57	25	346
		R: 5′-TGCCTGGGGCTTTTTCCCGA-3′
GalT-I	NM_001031661	F: 5′-CATGCACGATGTGGACCTAC-3′	59	25	147
		R: 5′-TGTTTGGACAGCAGCAGAAT-3′
GalT-II	NM_001106699	F: 5′-GCTTGGCAACTCTGCGACTA-3′	57	25	135
		R: 5′-GCCCAGCGATACATCTTCAC-3′
GlcAT-1	NM_001128184	F: 5′-CACACACCTGGCTGTCCTTA-3′	59	25	147
		R: 5′-GAGTCCTTCTCTCCCCCTACA-3′
CSgalnact-1	NM_001107309	F: 5′-TGGAAGGGAAGTAATGTCGT-3′	51	25	159
		R: 5′-ATGATGGCCGTAGATTATGC-3′
Aggrecanase-1	NM_023959	F: 5′-TCGCTTCGCTGAGTAGATTCGT-3′	55	25	141
		R: 5′-TTCGGATGCTTGGATGCTTAA-3′
Aggrecanase-2	NM_198761	F: 5′-CTGCGCTGTGATTGAAGATGAT-3′	59	20	158
		R: 5′-TGCTGGTAAGGATTGAAGACATT-3′
MMP-3	NM_133523	F: 5′-TGGCAGTGAAGAAGATGCTG-3′	61	25	167
		R: 5′-GCTTCCCTGTCATCTTCAGC-3′
GAPDH	NM_017008	F: 5′-GGCTCTCTGCTCCTCCCTGT-3′	59	20	123
		R: 5′-GTAACCAGGCGTCCGATACGGC-3′

PCR: polymerase chain reaction; Col2a1: collagen type II a 1; XylT-I: xylosyltransferase I; GalT-I: β1,4-galactosyltransferase 7; GalT-II: β1,3-galactosyltransferase 6; GlcAT-1: β1,3-glucuronosyltransferase I; CSgalnact-1: N-acetylgalactosaminyltransferase 1; MMP-3: matrix metallopeptidase 3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.