Subtoxic Levels of Apigenin Inhibit Expression and Secretion of VEGF by Uveal Melanoma Cells via Suppression of ERK1/2 and PI3K/Akt Pathways
Figure 6
Effects of Akt and MAPK inhibitors on the constitutive secretion of VEGF by uveal melanoma cells. Cells were plated into 24-well plates. Various MAPK and Akt inhibitors were added to the medium separately, including LY294002 (PI3K/Akt inhibitor), UO1026 (ERK inhibitor), SP600125 (JNK inhibitor), and SB203580 (p38 MAPK inhibitor) at a final concentration of 10 μM. After 24 h incubation, culture medium was collected, the VEGF levels were measured with human VEGF Quantikine ELISA kit and expressed as the percentages of the controls (cells cultured without any signal inhibitors). Akt and ERK inhibitors significantly decreased, whereas JNK and p38 inhibitors did not affect the VEGF levels in the conditioned culture medium from uveal melanoma cells. Data are mean ± SD (). , versus control (cells cultured without inhibitor).
