Ethanol Extract of Antrodia camphorata Grown on Germinated Brown Rice Suppresses Inflammatory Responses in Mice with Acute DSS-Induced Colitis

Figure 1

Effect of the CBR extract on NO and PGE₂ production_, and iNOS and COX-2 protein expression levels in LPS-stimulated RAW264.7 macrophages. Cells were incubated for 20 h with 1 𝜇g/mL of LPS in the absence or presence of the CBR or AC extract. The CBR extract was added 1 h prior to incubation with LPS. (a, b) Nitrite and PGE₂ production in the medium was determined using the Griess reagent and PGE₂ EIA kit. (c) Protein expression of iNOS and COX-2 was analyzed by western blot analysis and quantified by densitometric analysis. Data show the mean ± standard error (S.E.) of 3 independent experiments. A one-way ANOVA was used for comparisons of multiple group means followed by Dunnett’s t-test (^#𝑃<0.001 versus control; ^∗𝑃<0.01, **𝑃<0.05, and ***𝑃<0.001 versus LPS-stimulated control; ^$𝑃<0.05 versus AC).

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