Ethanol Extract of Antrodia camphorata Grown on Germinated Brown Rice Suppresses Inflammatory Responses in Mice with Acute DSS-Induced Colitis
Figure 7
Effect of the CBR extract on the DSS-induced iNOS and COX-2 protein expression and TNF-α and IL-6 mRNA expression levels. (a) Immunoblotting analysis of iNOS and COX-2. Representative colon tissue lysates from normal mice administered with drinking water (control), mice administered with DSS-induced colitis (DSS), and mice coadministered with DSS and CBR extract (DSS+CBR) (left to right lane). Protein expression of iNOS and COX-2 was analyzed by western blot analysis and quantified by densitometric analysis. (b, c) TNF-α and IL-6 mRNA expression levels of colon tissues were analyzed by RT-PCR and determined by quantitative RT-PCR by using GAPDH mRNA as the internal control. Data represent the mean standard error (S.E.) of 3 independent experiments. A one-way ANOVA was used for comparisons of multiple group means followed by Dunnett’s t-test (# versus control; *** versus DSS).
