Specific Dioscorea Phytoextracts Enhance Potency of TCL-Loaded DC-Based Cancer Vaccines
Figure 3
Phenotypic changes in DsII-TN5-treated DCs. (a) The quality of BMDCs was measured by expression of CD11c. On day 7 of cell culture, DCs were cultured in media supplemented with or without 1 μg/mL LPS for 24 h. The CD11c+ cell population of DCs was monitored by flow cytometry and DC morphology observed by microscopy. (A) The CD11c+ cell population was detected to be 85.2% (blue line) in immature DCs (without LPS treatment) and was detected to be 97% (red line) in mature DCs (with LPS treatment). Morphological characteristics of (B) immature DCs and (C) mature DCs at 400x magnification. (b) Phenotypic changes in (A) CD40, (B) CD80, and (C) CD86 cell-surface marker expressions on TCL-loaded DCs in response to treatment with DsII-TN5 at 50–1000 μg/mL doses. The -axis shows the mean fluorescence intensity (MFI). Data represent the mean ± SD obtained from three independent experiments.
