Trypanocidal Activity of Thioamide-Substituted Imidazoquinolinone: Electrochemical Properties and Biological Effects
Figure 4
Effect of C1 on cell death and state of the mitochondria evaluated by flow cytometry and Western blot. Epimastigotes of T. cruzi were treated with C1 22.5 μM during 8, 24, and 36 h. Parasites were (a) stained by Annexin V-FITC/PI, (b) stained by DIOC6, or (c) incubated with digitonin, to obtain the cytosolic and mitochondrial fractions, which were subjected to Western-blot analysis for cytochrome c. The numbers correspond to 1: untreated cells and 2, 3, and 4: treated cells with C1 during 8, 24, and 36 h, respectively. Methodology and data analysis were carried out as described in Section 2.
