Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MS/MS, Electron Microscope, and Congo Red Staining

Table 1

The analytical conditions of LC-MS/MS for the identification of the four constituents.

Constituents	Molecular weight	RT (min)	Mass fragments		Collision energy (eV)
			Q1 Mass (amu)	Q3 Mass (amu)
Saikosaponin A	780.98	4.31	781.40 [M + H]^+	455.35	−25.0
Saikosaponin D	780.98	5.33	781.40 [M + H]^+	455.35	−25.0
Ferulic acid	194.18	1.73	193.00 [M − H]^−	134.00	15.0
Paeoniflorin	480.46	1.24	498.20 [M + NH4]^+	179.10	−25.0