Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MS/MS, Electron Microscope, and Congo Red Staining

Table 2

Linear ranges, calibration curves, correlation coefficients (𝑟²), and detection limits of four constituents using LC-MS/MS.

Constituents	Linear range (ng/mL)	Calibration curve	𝑟^2	LOD (S/N = 3) (ng/mL)
Saikosaponin A	25–1000	𝑦=296.6𝑥−41.67	0.998	10
Saikosaponin D	50–1000	𝑦=245.1𝑥+755.8	0.997	10
Ferulic acid	50–1000	𝑦=472.8𝑥−8820	0.999	5
Paeoniflorin	50–1000	𝑦=553.8𝑥−1745	0.999	5