Immunomodulatory Effect of Chinese Herbal Medicine Formula Sheng-Fei-Yu-Chuan-Tang in Lipopolysaccharide-Induced Acute Lung Injury Mice
Figure 3
SFYCT reduced cytokine expression and NF-κB phosphorylation in LPS-challenged mice. (a) Inflammatory cytokine (left panel), TNFα, IL-1β, and IL-6 concentrations, and anti-inflammatory cytokine (right panel), IL-4 and IL-10 concentration of BALF in normal or LPS-challenged mice combined with PBS or SFYCT treatment ( in each group). Data are mean (SD). versus control group. versus LPS group. BALF was collected 24 h after the LPS challenge. Lung was collected 24 h after the LPS challenge. (b) Phosphorylation of NF-κB (Ser536) and iNOS expression were determined using Western blotting. The ratios of pNF-κB to NF-κB and iNOS to β-actin are shown, respectively. Data are representative of three individual experiments. (c) Expression of mRNA of TNFα and MCP-1 was analyzed using real-time RT-PCR ( in each group). Data are mean (SD). versus control group. versus LPS group.
