Immunomodulatory Effect of Chinese Herbal Medicine Formula Sheng-Fei-Yu-Chuan-Tang in Lipopolysaccharide-Induced Acute Lung Injury Mice

Figure 6

SFYCT reduced cytokine expression, NF-κB phosphorylation, and iNOS expression in LPS-stimulated macrophage. RAW264.7 cells (1×10⁶ cells/well in 6-well culture plates) were treated with LPS (3 μg/mL) for 2 hr with or without SFYCT at indicated concentration for 0.5 hr. (a) Phosphorylation of NF-κB (Ser536) and iNOS expression were determined using Western blotting. The ratios of pNF-κB to NF-κB and iNOS to β-actin are shown, respectively. Data are representative of three individual experiments. (b) Expression of mRNA of TNFα and MCP-1 was analyzed using real-time RT-PCR (𝑛=6). Data are mean (SD). ^#𝑃<0.05versus control group. ^∗𝑃<0.05versus LPS group.

(a)

(b)