Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages

Figure 8

Effect of EEP-B on cytokines production in LPS + IFN-γ stimulated J774A.1 macrophages: (a) IL-1α, (b) IL-1β, (c) IL-3, (d) IL-4, (e) IL-5, (f) IL-6, (g) IL-9, (h) IL-10, (i) IL-12p40, (j) IL-13, (k) IL-17, (l) TNF-α, (m) IFN-γ, (n) G-CSF, (o) GM-CSF, (p) MCP-1, (q) MIP-1α, (r) MIP-1β, and (s) RANTES. J774A.1 cells were incubated with 25–50 μg/mL EEP-B and/or LPS + IFN-γ for 24 h. Cytokine concentrations in the culture medium were determined by Multiplex (19-plex) bead-based cytokine assay. The values represent mean ± SD of two independent experiments (𝑛=8) * = 𝑃<0.05, ** = 𝑃<0.01, *** = 𝑃<0.001 compared to LPS + IFN-γ-stimulated cells.

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