Andrographis paniculata Extract and Andrographolide Modulate the Hepatic Drug Metabolism System and Plasma Tolbutamide Concentrations in Rats

Table 4

Effects of ethanolic extract of Andrographis paniculata (APE) and andrographolide (AND) on glucose tolerance test results in obese mice.

Plasma glucose (mg/dL)
Time (h)	0	0.25	0.5	1	2	4	8
Control (𝑛=6)	104±18^b	225±66^b	164±44^b	111±18	105±27	105±29^b	105±10^b
APE (𝑛=6)	77±9^a	155±26^a	115±24^a	87±25	87±16	98±13^b	103±11^b
AND (𝑛=8)	80±7^a	152±37^a	124±28^ab	99±29	88±28	76±17^a	80±17^a

High-fat diet-induced obese mice were orally dosed daily with 10 mL/kg of 0.5% aqueous methyl cellulose, 2 g/kg of APE, or 50 mg/kg of AND for 5 consecutive days. After the 5th daily dose, the animals were deprived of food overnight. On day 6, a 20 mg/kg dose of tolbutamide was orally administered to each animal. Thirty minutes later, a 1 g/kg oral dose of glucose, as a solution in water, was administered to each mouse. Serial blood samples up to 8 h after the glucose dose were collected from each mouse, and plasma glucose concentrations were determined. The values represent the mean ± SD, 𝑛=5. The significance of the difference among group means was determined by one-way ANOVA followed by Tukey’s test. ^abValues of treatments not sharing the same letter differ significantly, 𝑃<0.05.