Effect of Uncaria tomentosa Extract on Apoptosis Triggered by Oxaliplatin Exposure on HT29 Cells

Figure 2

Apoptosis was assessed using PE Annexin V Apoptosis Detection Kit I (BD Pharmingen). The cells were treated with the following concentrations of cytotoxic agent for 48 h or 72 h: (A) = control: culture medium without Uncaria tomentosa extract or oxaliplatin; (B) = chemotherapy: oxaliplatin 20 μmol/L; (C) = Uncaria: Uncaria tomentosa extract 750 μg/mL; (D) = Uncaria + chem.: Uncaria tomentosa extract 750 μg/mL + oxaliplatin 20 μmol/L. A total of 10,000 events were acquired and analyzed using FACSCalibur with CELLQuest software. Cells PE Annexin V and 7-AAD negative were considered viable cells; live-gated cells within the Annexin-V⁺7AAD⁻ compartment were identified as early apoptotic cells and gated cells within the Annexin-V⁺7AAD⁺ compartment were identified as late apoptotic/dead cells.