Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala

Figure 4

Cytotoxicity on splenocytes and inhibition on ConA-induced splenocyte proliferation of five procyanidin compounds 1–5. (a) Cytotoxicity of procyanidin compounds on BALB/c mice splenocytes. The cells were incubated with different concentration of procyanidin compounds for 48 h. And the cell viability was measured by MTT assay. (b) Inhibition of procyanidin compounds on ConA-induced splenocyte proliferation. BALB/c mice splenocytes (4 × 10⁵ cells/well) were stimulated by ConA (2 μg/mL) for 48 h in the presence of different procyanidin compounds. Cells were then pulsed with 0.25 μCi [³H]thymidine 8 h before the end of the experiment and were assessed for [³H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. , , treatment group versus control.

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