Gastrodiae Rhizoma Ethanol Extract Enhances Pentobarbital-Induced Sleeping Behaviors and Rapid Eye Movement Sleep via the Activation of GABA_A-ergic Transmission in Rodents

Figure 6

The effects of GREE on chloride influx in primary cultured cerebellar granule cells. After culturing cerebellar granule cells for 8 days, the cells were incubated with MQAE overnight, and then GREE (0.1, 1, and 10 μg/mL) and pentobarbital (PENT 10 μM) were added 1 h prior to measurement. Each column represents the mean with S.E.M. (). The significance of the effects of the compounds was assessed using analysis of variance (ANOVA). Where there was significant variability, the individual values were compared using Holm-Sidak test. , , compared with that of the control.