A Platform for Screening Potential Anticholinesterase Fractions and Components Obtained from Anemarrhena asphodeloides Bge for Treating Alzheimer’s Disease

Table 1

The effects of the tested extracts on AChE and BuChE inhibitory activities.

Solvents	AChE inhibition (%)			BuChE inhibition (%)
	Ultrasonic method	Heat reflux method	Cold soak method	Ultrasonic method	Heat reflux method	Cold soak method
Petroleum ether	—	—	—	—	—	1.6 ± 0.1
Dichloromethane	—	—	—	41.5 ± 0.5	15.7 ± 0.7	41.2 ± 0.6
Ethyl acetate	—	—	—	44.1 ± 0.5	27.7 ± 0.4	40.8 ± 0.8
Acetone	—	—	—	38.8 ± 0.3	22.2 ± 0.3	37.9 ± 0.7
Methanol	—	—	—	12.3 ± 0.4	5.4 ± 0.2	7.1 ± 0.3
95% ethanol	—	—	—	4.9 ± 0.2	2.8 ± 0.1	3.9 ± 0.3
70% ethanol	—	—	—	1.8 ± 0.1	—	1.6 ± 0.2
50% ethanol	—	—	—	2.8 ± 0.1	—	0.5 ± 0.1
30% ethanol	—	—	—	2.4 ± 0.1	—	1.8 ± 0.2
Water	—	—	—	—	—	—

AChE inhibition ratio of donepezil: 98.0 ± 0.5; BuChE inhibition ratio of donepezil: 80.2 ± 0.6.