Herbal Prescription, DSGOST, Prevents Cold-Induced RhoA Activation and Endothelin-1 Production in Endothelial Cells
Figure 4
DSGOST inhibition of cold-induced endothelin-1 expression. (a) HDMEC were pretreated with DSGOST (100 μg/mL) for 30 minutes and then exposed to cold (25°C) for another 4 hours. Relative endothelin-1 mRNA expression level was examined using real-time PCR, . (b) HDMEC were transfected with CA-RhoA for 48 hours and then treated with DSGOST (100 μg/mL) for 4 hours. Relative endothelin-1 mRNA level was detected using real-time PCR. (c) Endothelin-1 level from HDMEC-cultured medium was measured by ELISA. Experiments were performed in triplicate. Bars indicate the mean ± SD, . (d) Schematic cartoon. DSGOST inhibits cold-induced RhoA activation, resulting in repression of endothelin-1 (ET-1) production from EC. Subsequently, ET-1-induced RhoA activation and α2c-adrenoreceptor (α2c-AR) translocation to the membrane are blocked in VSMC. In addition, DSGOST may directly inhibit cold-induced responses in VSMC.
