Effect of Eclipta prostrata on 11Beta-Hydroxysteroid Dehydrogenase in Rat Liver and Kidney

Table 1

Primer sequences used for real-time polymerase chain reaction.

Gene	5′-3′ sequence	PCR condition
11β-HDS I		Stage 1: Predenaturation; (30 s at 95°C); 1 Cycle
Forward; Reverse	TGACCAAGGTCAACGTGTCCA; ATGATCTCCAGGGCGCATTC
11β-HDS II
Forward; Reverse	GACCTTAGCCCCGTTGTAGATG; GGCAGGTAGTGGTGGATGAAA	Stage 2: PCR reaction; (5 s at 95°C); (20 s at 60°C); (60 s at 40°C); 40 Cycle
β-Actin
Forward; Reverse	GGAGATTACTGCCCTGGCTCCTA; GACTCATCGTACTCCTGCTTGCTG