Pien Tze Huang Overcomes Multidrug Resistance and Epithelial-Mesenchymal Transition in Human Colorectal Carcinoma Cells via Suppression of TGF-β Pathway

Figure 3

Effect of MDR on the morphological changes, the expression of ABCG2, EMT-related factors, and the activation of TGF-β pathway. (a) Morphology of HCT-8/5-FU and parental HCT-8 cells was observed using phase-contrast microscopy. The photographs were taken at a magnification of 400x. Images are representative of three independent experiments. (b-c) The mRNA and protein expression levels of ABCG2, E-cadherin, N-cadherin, ZEB1, ZEB2, TGF-β, and SMAD4 in parental HCT-8 and HCT-8/5-FU cells were determined by RT-PCR and Western Blot analyses. GAPDH or β-actin was used as the internal control for RT-PCR or Western Blot, respectively. Images are representatives of three independent experiments.

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