Ethyl Acetate Extract of Artemisia anomala S. Moore Displays Potent Anti-Inflammatory Effect
Figure 4
Effect of EAFA on total oxidant activity and iNOS expression in LPS/IFN-stimulated RAW264.7 cells. (a) Cells were plated at a density of cells/well in 30 mm dishes and allowed to attach overnight. EAFA was added 1 h prior to the treatment of IFN (10 U/mL) plus LPS (100 ng/mL) for 6 h. Whole cell lysates were analyzed by FRAP assay and standardized by protein concentration. The data shown are representative of three independent experiments. ##; # versus control group; **; * versus model group. (b) RAW264.7 cells ( cells/dish) were pretreated with varying concentrations of EAFA for 1 h followed by LPS (100 ng/mL) and IFN- (10 U/mL) treatment for 4 h. Total RNA was isolated and subjected to qRT-PCR. -actin mRNA was used as an internal control for standardization. (c) RAW264.7 cells were plated at a density of cells in 30 mm dish for overnight. EAFA was added 1 h prior to the treatment of IFN (10 U/mL) plus LPS (100 ng/mL) for 6 h. Whole cell lysates were prepared and subjected to western blotting. The data shown are representative of three independent experiments. ##, # versus control group; **, * versus model group.
