Wen-Dan Decoction Improves Negative Emotions in Sleep-Deprived Rats by Regulating Orexin-A and Leptin Expression

Table 2

Primer sequences, length of PCR products, and optimal annealing temperature for each gene used in real-time quantitative PCR.

Primer	Sequence (5′-3′)	𝑇𝑎 (°C)	bp
GAPDH	F: 5′GGAAAGCTGTGGCGTGAT3′; R: 5′AAGGTGGAAGAATGGGAGTT3′	60	308
Orexin-A	F: 5′CGCCAGAAGACGTGTTCCT3′; R: 5′GCCGCTTTCCCAGAGTGAG3′	60	88
Orexin receptor 1	F: 5′TTTCGGGAGCAGTTCAAGG3′; R: 5′CCCCAGGCAAAGGATCAA3′	60	203
OB-R	F: 5′GCAGTCCAGCCTACACTCTTG3′; R: 5′GCTTCACCACATACCTCCTCAC3′	60	171