Wen-Dan Decoction Improves Negative Emotions in Sleep-Deprived Rats by Regulating Orexin-A and Leptin Expression

Table 4

The effect of sleep deprivation on the mean integrated optical density (MOD) of orexin-A and leptin in the prefrontal cortex and hypothalamus and the regulation of WDD.

Part	Groups	Orexin-A	Leptin
Prefrontal cortex	Control group	0.17 ± 0.03	0.40 ± 0.02
	Model group	0.14 ± 0.02**	0.19 ± 0.04**
	Diazepam group	0.14 ± 0.01**	0.22 ± 0.03^**▲
	Wen-dan group	0.16 ± 0.01^▲▲**	0.27 ± 0.03^**▲▲
Hypothalamus	Control group	0.17 ± 0.01	0.27 ± 0.03
	Model group	0.12 ± 0.02**	0.15 ± 0.04**
	Diazepam group	0.13 ± 0.02**	0.18 ± 0.03^**▲▲
	Wen-dan group	0.14 ± 0.01^**▲▲★	0.21 ± 0.02^**▲▲★★

^∗∗𝑃<0.01: versus the control group; ^▲𝑃<0.05: versus the model group; ^▲▲𝑃<0.01: versus the model group; ^★𝑃<0.05: versus the diazepam group; ^★★𝑃<0.01: versus the diazepam group. Data is presented as mean ± standard error of the mean (SEM).