Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line

Table 5

Effects of Chinese compound recipes on ERBB2 and ESR1 gene expression.

Names	Abbreviation	Dose range (μg/mL)	ERBB2 (fold of control)			ESR1 (fold of control)
			≦1.5	1.5–2	≧2	≦1.5	1.5–2	≧2
Si-Wu-Tang	SWT	1, 3, 10		10*				1, 3, 10
Jia-Wei-Xiao-Yao-San	JWXYS	1, 3, 10
Suan-Zsao-Ren-Tang	SZRT	0.1, 0.3, 1			0.1, 0.3, 1		0.1, 0.3, 1
Reduced formula of Suan-Zsao-Ren-Tang^#	r-SZRT	0.1, 0.3, 1				0.3	0.1, 1
Kuan-Shin-Yin	KHY	0.3, 1, 3						0.3, 1, 3
VGH-S4	VGH-S4	0.1, 0.3, 1			0.3, 1			0.1, 0.3, 1

ERBB2 and ESR1 gene expression was determined by luciferase reporter assay as described in the Methods section. VGH-S4 is a compound recipe commonly used in Taipei Veterans General Hospital.
^#Reduced formula of Suan-Zsao-Ren-Tang (r-SZRT) is composed of those materials in Suan-Zsao-Ren-Tang except for Ligusticum chuanxiong which is absent.
*Number indicates the dose that has effect with statistically significant difference (𝑃<0.05) versus vehicle group (𝑛≥6).