A Cistanches Herba Fraction/β-Sitosterol Causes a Redox-Sensitive Induction of Mitochondrial Uncoupling and Activation of Adenosine Monophosphate-Dependent Protein Kinase/Peroxisome Proliferator-Activated Receptor γ Coactivator-1 in C2C12 Myotubes: A Possible Mechanism Underlying the Weight Reduction Effect

Figure 2

Effects of BSS on mitochondrial membrane potential in C2C12 myotubes. Cells were prestained with JC-1 at 37°C for 10 min. The measurement of mitochondrial membrane potential was initiated immediately after the addition of the test compounds. Data were analyzed by mixed-design analysis of variance and are expressed as percent control with respect to the non-HCF1 incubated time-match controls. Values given are means ± SEM, with ; different from non-drug preincubated time-match controls (0 to 10 min); different from non-drug preincubated time-match controls (11 to 60 min); different from HCF1 preincubated time-match controls (0 to 10 min); different from HCF1 preincubated time-match controls (11 to 60 min) ().

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