Huaier Aqueous Extract Induces Hepatocellular Carcinoma Cells Arrest in S Phase via JNK Signaling Pathway

Figure 4

Effects of Huaier extract on expression of procaspase 3, cleaved caspase 3, and cleaved PARP in HCC cells. HCC cells were treated with various concentrations (0, 2, 4, 8, and 16 mg/mL) of Huaier extract for 48 h, and then cell lysates were subjected to Western blotting with procaspase 3, cleaved caspase 3, and cleaved PARP antibodies. The densitometric ratios were normalized to those of tubulin, and the results are expressed as the mean densitometric ratios ± SD in three independent experiments. , , and , compared with that of the untreated control, respectively (0 mg/mL).