The Flavonoid Apigenin Ameliorates Cisplatin-Induced Nephrotoxicity through Reduction of p53 Activation and Promotion of PI3K/Akt Pathway in Human Renal Proximal Tubular Epithelial Cells

Figure 3

The effects of apigenin on cisplatin-induced caspase-3 activation and PARP cleavage in HK-2 cells. Cells were pretreated with different concentrations of apigenin for 1 h and then exposed to 40 μM of cisplatin for 24 h. Caspase-3 activity was measured using a caspase-3 colorimetric assay kit. Values are means ± SD, . versus cells treated with cisplatin alone (a). The levels of cleaved caspase-3 and PARP were examined by Western blot analysis using anticleaved caspase-3 (p20) and anti-PARP antibodies. The density of band was quantified by a densitometry using Bio-Rad Quantity One software. Value are means ± SD, . versus cells treated with cisplatin alone (b).

(a)

(b)