Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells

Figure 5

Expression of differentiation-associated cell cycle regulators after 72 h of 4ME treatment. (a) pRb phosphorylation on Ser 780 was reduced after 72 h of 4ME treatment, as determined by western blot analysis. (b) Following 72 h of challenge, 4ME increases p38 phosphorylation in all concentrations used. Data representative of two other experiments are shown. Panel (c) shows quantification of p38 expression at protein level in THP-1 cells after 72 h of 4ME treatment as determined by western blot analysis. Values shown are mean ± SD of two independent experiments performed in duplicate.

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