Research Article
[Retracted] Sagunja-Tang Improves Lipid Related Disease in a Postmenopausal Rat Model and HepG2 Cells
Figure 4
Effects of SGJT on lipid accumulation, total cholesterol, and LDL/VLD levels in HepG2 cells. HepG2 cells were treated with 30 μM simvastatin or several concentrations of SGJT (250–1,000 μg/mL) with 20 mg/mL MβCD for 8 h in 0.2% BSA-DMEM. Lipid accumulation was visualized by Oil Red O staining (×600 magnification) and quantitative analysis of lipid deposition in cells was analyzed by spectrophotometer (a). Total intracellular cholesterol (b) and LDL/VLDL (c) levels were measured by ELISA. The data were mean ± SD from three samples for each group. ##Significantly different between the control and MβCD treatment at (two-sample -test). Values not sharing a common alphabet as superscripts are significantly different from each other at the level of (ANOVA followed by Duncan’s test). Similar results were obtained in three separate experiments.
(a) |
(b) |
(c) |