Auraptene, a Major Compound of Supercritical Fluid Extract of Phalsak (Citrus Hassaku Hort ex Tanaka), Induces Apoptosis through the Suppression of mTOR Pathways in Human Gastric Cancer SNU-1 Cells

Figure 4

Western blot analysis of apoptosis and PI3K/Akt/mTOR related protein expression. (a) Cells were lysed after incubation with different concentrations of auraptene (25–100 μM). After 24 h, cell lysates were subjected to western blotting as mentioned in the method. (b) Western blotting for the effect of auraptene on Akt/mTOR related proteins in SNU-1 cells. (c) Cells were treated with rapamycin for 24 h in SNU-1 cells. β-actin was used as an internal control. (d) Cell growth inhibition by rapamycin. Viability was determined on the basis of MTT reduction assay against SNU-1 cells for 24 and 48 h. (Data represent the means SD of at least four independent experiments. indicate statistically significant differences versus control group.)

(a)

(b)

(c)

(d)