Myelinated Afferents Are Involved in Pathology of the Spontaneous Electrical Activity and Mechanical Hyperalgesia of Myofascial Trigger Spots in Rats

Figure 1

Modified pyridine-silver staining and immunohistochemistry for NF 200. (a–e) show modified pyridine-silver staining results. In the longitudinal views of MTrSs (a) and non-MTrSs (b), nerve endings are dark brown or black fibers in muscular fasciae along the muscle fibers, and in transverse views, they are dark brown or black spots between myocytes. More stained fibers exist in MTrSs (c), and fewer in non-MTrSs (d). (e) presents an illustrative diagram for modified pyridine-silver staining results, and “” means . (f–j) show immunohistochemistry results for NF 200. In the longitudinal views of MTrSs (f) and non-MTrSs (g), NF 200 positive myelinated afferent nerve endings are brown fibers along the muscle fibers, while in the transverse views, they are brown spots between myocytes. More positive fibers exist in MTrSs (h), and fewer exist in non-MTrSs (i). (j) presents an illustrative diagram for immunohistochemistry results, and “#” means . Scale bar = 20 μm.