Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway

Figure 5

The effect of SCU and PKG inhibitor in rats with CIR treatment. (a) Representative images of triphenyl tetrazolium chloride (TTC) staining of ischemic brain slices from CIR (ischemia 60 minutes/reperfusion 24 hours) rats with SCU treatment. (b) Quantification of the corrected cerebral infarct volume in CIR rats with SCU treatment. rats in each group. One-way ANOVA followed by SNK test versus model, , . (c) Cumulative concentration response curves of acetylcholine chloride (ACh) in isolated basilar artery (BA) segments from normal or CIR rats which were given SCU (90 mg/kg, i.v.) or PKG inhibitor treatment. Relaxation in response to ACh is expressed as a percent of precontraction with U46199 (1 μmol/L). segments obtained from 3 rats with different treatments. Two-way ANOVA test versus model, , versus SCU, . (d) Representative images of TTC staining of ischemic brain slices from CIR (ischemia 60 minutes/reperfusion 24 hours) rats, with SCU (90 mg/kg i.v.) and PKG inhibitor (Rp-8-Br-cGMPS, 50 μg/kg i.v.) alone and combined (SCU 90 mg/kg + Rp-8-Br-cGMPS 50 μg/kg) treatments. (e) Quantification of the corrected cerebral infarct size in CIR rats administered with SCU and PKG inhibitor. rats in each group. One-way ANOVA followed by SNK test versus model, , versus SCU, . Data are means ± SEM.