Suppression of Propionibacterium acnes-Induced Dermatitis by a Traditional Japanese Medicine, Jumihaidokuto, Modifying Macrophage Functions

Table 1

Effects of jumihaidokuto-related compounds on macrophage functions.

Test compound	IFN-γ (10 ng/mL)	Expression of activation marker		Phagocytosis
		CD86	CD192	% of FITC^+ cells	MFI
—	−	0.08 ± 0.06	0.19 ± 0.16	14.43 ± 1.08	3.16 ± 0.17
—	+	1.70 ± 0.07	1.57 ± 0.05	20.86 ± 1.05	4.76 ± 0.28
Liquiritin	+	3.28 ± 0.10	3.76 ± 0.05	24.67 ± 1.24	6.07 ± 0.41
Liquiritigenin	+	3.47 ± 0.12	3.80 ± 0.19	31.01 ± 3.06	7.88 ± 0.93
Isoliquiritin	+	5.27 ± 0.17	4.21 ± 0.15	42.42 ± 1.24	12.94 ± 0.71
18β-Glycyrrhetinic acid	+	1.84 ± 0.13	2.24 ± 0.12	18.00 ± 1.45	4.18 ± 0.31
Cimifugin	+	3.82 ± 0.43	4.84 ± 0.65	19.05 ± 0.59	4.28 ± 0.10
4-O-Methylgallic acid	+	1.18 ± 0.10	1.18 ± 0.12	18.91 ± 1.36	4.27 ± 0.31

Human monocytic THP-1 cells were seeded in 96-well culture plates at 1 or 2 × 10⁴ cells/well and cultured with test samples (30 μmol/L) in the presence or absence of 10 ng/mL human IFN-γ. To examine the activation markers, cells were harvested 2 d later and stained with a fluorescent-labeled antibody specific to CD86 or CD192. Their expressions in cells were determined by flow cytometry. Data are shown as mean fluorescence intensity (MFI) subtracted with MFI of naive cells (CD86: 7.29, CD192: 11.60). In the study of phagocytosis, FITC-microspheres were added at 6 × 10⁶ beads/well after the preculture for 3 d and cultured for an additional 2 h. Cells were harvested, and the FITC-positive cells were measured. . versus IFN-γ alone control (Dunnett’s test).