Mechanistic Study of the Phytocompound, 2-<i>β</i>-D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches

<div>Effect of GHTT on mitochondrial membrane potential (a) and apoptosis (b) in Jurkat cells. (a) Jurkat cells were incubated with TMRM. After washing, the cells were treated with GHTT, DMSO, a negative control, and tunicamycin (Tm, 10 <i>μ</i>g/mL), a positive control, followed by flow cytometry analysis. (b) Jurkat cells were incubated with GHTT and DMSO. After washing, the cells were stained with PI and annexin V and subjected to flow cytometry analysis. (c) Total lysates of Jurkat cells treated with GHTT and DMSO underwent SDS-PAGE. Following protein transfer, the membrane was blotted with the indicated antibodies.</div>

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Figure 4

Figure 4: Mechanistic Study of the Phytocompound, 2-<i>β</i>-D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches 

Figure 4 | Mechanistic Study of the Phytocompound, 2-β-D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches 