The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus

Figure 4

Combined effect of ATPase inhibitors on the susceptibility of MRSA (ATCC 33591) to shikonin (SKN). Bacterial viability was determined by the absorbance at 600 nm after incubation for 24 h with SKN (7.8 μg/mL, 0.5 MIC value), 0.01% NaN₃ (NA, (a)), and 0.005% N,N′-dicyclohexylcarbodiimide (DCCD, (b)), or their combinations SKN + NA and SKN + DCCD, respectively. The data are presented as the mean ± S.D. of three independent experiments. compared to SKN alone. CON, untreated control MRSA.

(a)

(b)