Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118

Table 1

Primer sequences and reaction conditions of RT-PCR.

Genes	Primers	Sequences (5′-3′)	Annealing temperature (°C)	Cycle number
NR024118	Forward	5′GCTGCCCACCTCACTCAC3′	63°C	40
	Reverse	5′CTTTATTGCTCCATTTCCCTC3′
SOCS3	Forward	5′TTCTTCACGCTCAGCGTCAAG3′	55°C	30
	Reverse	5′ATGTAATAGGCTCTTCTGGGG3′
IL-6	Forward	5′GGCTGCTTCTGGTGATGG3′	55°C	30
	Reverse	5′AGAGATTTTGCCGAGGATGTA3′
IL-8	Forward	5′GCCAAGGAGTGCTAAAGAACTTAGA3′	58°C	30
	Reverse	5′ATTTCTGTGTTGGCGCAGTGT3′
MMP-1	Forward	5′AGGGTCAAGCAGACATCA3′	56°C	30
	Reverse	5′CAGAAGGGCAAGCATTAG3′
MMP-3	Forward	5′CCTGCTTTGTCCTTTGATGC3′	55°C	30
	Reverse	5′TGAGTCAATCCCTGGAAAGTC3′
β-actin	Forward	5′TGACGGGGTCACCCACACTGTGCCCATCT3′	60°C	30
	Reverse	5′CTAGAAGCATTTGCGGTGGACGATG3′