Validation of Quantitative HPLC Method for Bacosides in KeenMind

Table 6

Results of method precision (1st operator) and intermediate precision (2nd operator).

Sample	Injection	Bacosides (%w/w)
replicate	replicate	1st operator	2nd operator
1	1	1.02609	0.9489
	2	1.0181	0.9501
	3	1.0053	0.9494
2	1	1.03001	1.047
	2	1.05028	0.9872
	3	1.03453	0.97095
3	1	1.00957	0.97228
	2	1.02717	0.97656
	3	1.05503	0.9754
4	1	0.99785	0.938
	2	0.9765	0.97555
	3	0.9665	0.9839
5	1	1.09557	0.9748
	2	1.03263	1.0428
	3	1.04265	1.01844
6	1	0.9804	1.0184
	2	0.97473	0.9899
	3	0.99676	0.99117
%w/w (mean)		1.018	0.984
STDEV		0.031	0.024
% RSD		3.00	2.46