A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells

Table 1

Bio-guided fractionation of CH₂Cl₂ extract and IC₅₀ of fractions on the NF-B driven transcription. AGS cells were transiently transfected with NF-B-luc plasmid and treated with TNF- in the presence of fractions (2.5–20 g/mL) for 24 h. NF-B driven transcription was proportional to luciferase signal measured. The inhibitory activity of fractions was considered significant if 50 g/mL.

Fraction	Hexane : AcOEt	IC50 (mean ± s.d.)
A	95 : 5	50 g/mL
B	90 : 10	50 g/mL
C	80 : 20	50 g/mL
D	70 : 30	23.01 ± 8.51 g/mL
E	60 : 40	50 g/mL
F	50 : 50	50 g/mL
G	40 : 60	50 g/mL
H	30 : 70	TOXIC
I	20 : 80	TOXIC
J	10 : 90	4.29 ± 0.97 g/mL
K	AcOEt	19.59 ± 5.77 g/mL
L	AcOEt	15.52 ± 5.21 g/mL
M	Acetone	12.84 ± 4.43 g/mL
N	Acetone	11.59 ± 3.26 g/mL
O	Act∖iPrOH 9 : 1	7.82 ± 2.13 g/mL
P	Act∖iPrOH 9 : 1	50 g/mL