Traditional Herbal Formula Banhasasim-tang Exerts Anti-Inflammatory Effects in RAW 264.7 Macrophages and HaCaT Keratinocytes

Figure 6

Effect of Banhasasim-tang (BHSST) on NF-κB activation in RAW 264.7 cells. Cells were pretreated with BHSST (250, 500 or 1000 μg/mL) for 2 h and then stimulated with LPS (1 μg/mL) for 30 min. (a) Protein expression of phospho-NF-κB p65 was analyzed by Western blotting. The relative abundance of protein was calculated as phospho-NF-κB p65/β-actin ratio. Values are expressed as mean ± SEM of three independent experiments. versus vehicle control cells; and versus LPS-treated cells.

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