Antiproliferative and Apoptosis Induction Potential of the Methanolic Leaf Extract of Holarrhena floribunda (G. Don)

Table 2

The percentage of cell populations in different stages (live, apoptotic, and necrotic) following the extract treatment and evaluated by double staining in Annexin V-FITC/propidium iodide using flow cytometric assay.

	HeLa			MCF-7			HT-29
	12 h	24 h	48 h	12 h	24 h	48 h	12 h	24 h	48 h
Live (%)	92.41 ± 0.03	84.94 ± 0.69	19.05 ± 0.51	95.22 ± 1.36	92.15 ± 1.77	7.1 ± 1.23	94.22 ± 2.23	78.45 ± 2.25	83.43 ± 4.61
Early apoptosis (%)	3.41 ± 0.32	6.26 ± 0.50	3.41 ± 0.36	4.69 ± 1.27	6.11 ± 0.34	0.15 ± 0.01	4.56 ± 1.60	16.72 ± 1.64	0.01 ± 0.01
Late apoptosis (%)	3.53 ± 0.12	6.53 ± 0.78	33.47 ± 1.90	0.18 ± 0.02	0.52 ± 0.19	3.93 ± 1.58	0.26 ± 0.04	4.46 ± 0.70	0.46 ± 0.16
Dead (%)	0.66 ± 0.23	1.38 ± 1.20	43.60 ± 1.75	0.03 ± 0.02	0.16 ± 0.03	87.60 ± 1.17	0.05 ± 0.01	0.07 ± 0.01	16.42 ± 1.73