[Retracted] Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro

Figure 4

Detection of germ cell and oocyte markers in germ cell-like cells and OLCs by real-time RT-PCR. Real-time RT-PCR analysis of expressions of germ cell markers (OCT4, SSEA-1, Stella, Blimp1, Prdm14, and Tfap2c) in the PGC-like cells after 7 days of differentiation and oocyte markers (SCP3, GDF9, ZP1, ZP2, and ZP3) after 24 days of treatment with the rat medicated serums containing common ingredients of ZGW/YGW (a), YGW (b), ZGW (c), and normal rat serum (d). Relative mRNA levels are normalized for the GAPDH housekeeper. The results are presented relative to the control cells (). Data represents the mean ± SEM of three independent experiments. * indicates statistical significance when compared to day 0 of the culture.

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