Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus acutissima Cortex

Figure 3

Suppression of testosterone-induced sebum synthesis of hamster primary sebocytes by BK treatment. Hamster-derived sebaceous gland cells (Ha-SE) were precultured, until confluent in the presence of epidermal growth factor, and further treated with the indicated concentrations of bokusoku (BK) and a differentiation factor, 10 μmol/L testosterone or 10 μg/mL insulin. Eight days later, the sebum synthesis was determined using a lipogenesis detecting assay kit. .