Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus acutissima Cortex

Figure 4

Representative images of hamster primary sebocytes treated with or without BK in the presence of a differentiation factor. Hamster-derived sebaceous gland cells (Ha-SE) were precultured until confluent in the presence of epidermal growth factor, and further treated with 10 μg/mL bokusoku (BK) or vehicle in the presence of a differentiation factor, 10 μmol/L testosterone or 10 μg/mL insulin. Eight days later, cells were stained with Oil Red O. The representative images of sebum droplets were shown for ((A), (a)) none, ((B), (b)) testosterone + vehicle, ((C), (c)) testosterone + BK, ((D), (d)) insulin + vehicle, and ((E), (e)) insulin + BK. Scale bars: 20 μm. The images shown in (A), (B), (C), (D), and (E) were enlargements of those shown in (a), (b), (c), (d), and (e), respectively.

(a)

(b)

(c)

(d)

(e)