Central Antinociceptive and Mechanism of Action of Pereskia bleo Kunth Leaves Crude Extract, Fractions, and Isolated Compounds

Figure 7

Effects of different antagonists on antinociceptive activity of E, H, EA, and B fractions of Pereskia bleo Kunth leaves in the hot plate model. Animals were pretreated with atropine (1 mg/kg, i.p.), L-NAME (3 mg/kg, i.p.), or naloxone (1 mg/kg, i.p.) 30 min prior to oral administration of fractions (100 mg/kg). The results are presented as mean ± S.D. (–10) of area under the curve calculated using Prism Software 5.0. Statistical significance was calculated by ANOVA followed by Bonferroni’s test. when comparing morphine- or fraction-treated mice with vehicle-treated group and when comparing antagonists treated mice with fractions-treated group.