Ointment of Brassica oleracea var. capitata Matures the Extracellular Matrix in Skin Wounds of Wistar Rats

Figure 2

Cellularity in scar tissue of rats treated with Brassica oleracea and Helianthus annuus. The top panels (a and b) are representative photomicrographs of the computational method of color segmentation to estimate cell distribution in scar tissue (c). Digital images of skin sections stained with hematoxylin and eosin (a, bar = 40 μm) were computationally modified using a color-cube-based mode and a new mask was applied by selecting all pixels of varying dark blue color levels. Then, a new image in black and white was generated after color segmentation (b, bar = 40 μm) and the number of cell nuclei (white color) was measured by point counting. Balsam: Brassica oleracea oil; ointment: Brassica oleracea lanolin; sunflower: Helianthus annuus oil; control: 0.9% saline solution. Data are represented as mean and standard deviation (mean ± SD). denotes statistical differences between groups (), versus control; Kruskal-Wallis test.

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