Bavachalcone Enhances RORα Expression, Controls Bmal1 Circadian Transcription, and Depresses Cellular Senescence in Human Endothelial Cells

Figure 5

Bavachalcone-delayed cellular senescence, partially through RORα-Bmal1 pathway. (a and b) HUVECs were passaged from the 15th to the 45th generation and treated with or without bavachalcone and with or without VPR-66, a RORα inhibitor. Subsequently, the cells were dyed using a β-galactosidase senescence assay kit. The percentage of senescent cells was obtained by counting more than 500 cells in each sample ( each). (c and d) HUVECs were passaged from the 20th to the 45th generation and treated with or without bavachalcone and with or without VPR-66. The mRNA expression levels were measured using real-time quantitative PCR ( each). Data are expressed as the mean ± SD. versus p15 or p20 passages vehicle control; versus p45 passage vehicle control; versus p45 passage bavachalcone treatment.

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