Research Article
Eucommia ulmoides Oliv. (Du-Zhong) Lignans Inhibit Angiotensin II-Stimulated Proliferation by Affecting P21, P27, and Bax Expression in Rat Mesangial Cells
Figure 1
Effect of lignans on expression of P21 and P27 in RMCs induced by Ang II. β-actin and GAPDH were used as an internal loading control gene. Both mRNA and protein relative expression levels were expressed as folds of control. All values were expressed as mean ± SD (). (a) Effect of lignans on mRNA expression of P21 and P27 by RT-qPCR. (b, c1~c2) Effect of lignans on protein expression of P21 and P27 by western blot; Con: control; Ang II: 10−8 mol/L Ang II group; Epa: 10−8 mol/L Ang II + 20 μmol/L epalrestat group; L Lig: 10−8 mol/L Ang II + 20 mg/L lignan group; M Lig: 10−8 mol/L Ang II + 40 mg/L lignan group; H Lig: 10−8 mol/L Ang II + 80 mg/L lignan group; , compared with the control group; , , compared with the 10−8 mol/L Ang II group.