Eucommia ulmoides Oliv. (Du-Zhong) Lignans Inhibit Angiotensin II-Stimulated Proliferation by Affecting P21, P27, and Bax Expression in Rat Mesangial Cells

Figure 3

Effect of lignans on expression of Bax and Bcl-2 in RMCs induced by Ang II. β-actin and GAPDH were used as an internal loading control gene. Both mRNA and protein relative expression levels were expressed as folds of control. All values were expressed as mean ± SD (). (a) Effect of lignans on mRNA expression of Bax and Bcl-2 by RT-qPCR. (b, c1~c2) Effect of lignans on protein expression of Bax and Bcl-2 by western blot. Con: control; Ang II: 10⁻⁸ mol/L Ang II group; Epa: 10⁻⁸ mol/L Ang II + 20 μmol/L epalrestat group; L Lig: 10⁻⁸ mol/L Ang II + 20 mg/L lignan group; M Lig: 10⁻⁸ mol/L Ang II + 40 mg/L lignan group; H Lig: 10⁻⁸ mol/L Ang II + 80 mg/L lignan group; , compared with the control group; , , compared with the 10⁻⁸ mol/L Ang II group.