Research Article
Eucommia ulmoides Oliv. (Du-Zhong) Lignans Inhibit Angiotensin II-Stimulated Proliferation by Affecting P21, P27, and Bax Expression in Rat Mesangial Cells
Figure 4
Effect of lignans on AR in RMCs induced by Ang II. GAPDH was used as an internal loading control gene. All values were expressed as mean ± SD (). (a) Effect of lignans on activity of AR; (b) effect of lignans on mRNA expression of AR by RT-qPCR; (c1, c2) effect of lignans on protein expression of AR by western blot. Con: control; Ang II: 10−8 mol/L Ang II group; Epa: 10−8 mol/L Ang II + 20 μmol/L epalrestat group; L Lig: 10−8 mol/L Ang II+ 20 mg/L lignan group; M Lig: 10−8 mol/L Ang II + 40 mg/L lignan group; H Lig: 10−8 mol/L Ang II + 80 mg/L lignan group; , compared with the control group; , , compared with the 10−8 mol/L Ang II group.