Eucommia ulmoides Oliv. (Du-Zhong) Lignans Inhibit Angiotensin II-Stimulated Proliferation by Affecting P21, P27, and Bax Expression in Rat Mesangial Cells

Table 2

Effect of lignans on the cell cycle in RMCs induced by Ang II.

Groups	G1 period (%)	G2 period (%)	S period (%)
Con	73.9 ± 0.6	13.5 ± 0.7	12.6 ± 0.2
Ang II	69.2 ± 0.3	8.4 ± 0.8	22.4 ± 1.1
Epa	76.4 ± 1.4	12.5 ± 1.1^#	11.1 ± 0.4^##
L Lig	73.5 ± 2.5	12.5 ± 2.2^#	14.0 ± 0.6^##
M Lig	76.9 ± 4.1	13.0 ± 4.0^#	10.1 ± 0.2^##
H Lig	77.6 ± 0.7^##	15.3 ± 1.3^##	7.1 ± 0.7^##

Note: RMCs were treated with Ang II (10⁻⁸ mol/L), epalrestat (20 μmol/L), and various concentrations of Eucommia lignans (20, 40, and 80 mg/L) for 48 h, and cell cycle was assessed by flow cytometry. Results were given in mean ± SD (). , compared with the control group; ^#, ^##, compared with the 10⁻⁸ mol/L Ang II group.